Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Gas Chromatography-Mass Spectrometric Analysis of Essential Oil of Aerial Parts of Glycosmis parviflora (Sims) Little (Rutaceae)

Zhi Long Liu¹ , Kai Yang¹, Peng Hua Bai¹, Ligang Zhou², Shao Liang Liu³, Qi Zhi Liu¹

¹Department of Entomology; ²Department of Plant Pathology, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193,China; ³Department of Biology, Faculty of Preclinical Medicine, GuangXi Traditional Chinese Medical University, Nanning 530001, China.

For correspondence:- Zhi Liu Email: zhilongliu@cau.edu.cn Tel:+861062732800

Received: 17 January 2013 Accepted: 6 January 2014 Published: 20 February 2014

Citation: Liu ZL, Yang K, Bai PH, Zhou L, Liu SL, Liu QZ. Gas Chromatography-Mass Spectrometric Analysis of Essential Oil of Aerial Parts of Glycosmis parviflora (Sims) Little (Rutaceae). Trop J Pharm Res 2014; 13(2):275-280 doi: 10.4314/tjpr.v13i2.17

© 2014 The authors.
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Abstract

Purpose: To investigate the chemical composition and toxicity of the essential oil of Glycosmis parviflora (Sims) Little aerial parts against root-knot nematode and two grain storage insects (maize weevil and red flour beetle).

Methods: Steam distillation of G. parviflora was carried out using Clavenger apparatus in order to extract its volatile oil. Gas chromatography/mass spectrometric (GC/MS) analysis (HP-5MS column) of the essential oil was performed and the toxicity of the oil determined by contact test.

Results: A total of 37 components comprising 98.7 % of the essential oil were identified, of which (Z)-caryophyllene (20.6 %), methyl isoeugenol (11.1 %), (Z)-β-ocimene (8.9 %), α-cubebene (6.4 %), nerolidol (5.4 %), aromandendrene (4.9 %) and γ-pyronene (4.7 %) were found to be the major components. The essential oil possessed strong nematicidal activity against M. incognita with an LC₅₀ value of 92.84 μg/ml. The essential oil of G. parviflora exhibited strong contact toxicity against S. zeamais and T. castaneum adults with LD₅₀values of 41.7 and 22.6 μg/adult, respectively.

Conclusion: The study indicates that the essential oil of G. parviflora aerial parts has a potential for development into a natural insecticide/nematicide for control of nematodes and grain storage insects.

Keywords: Glycosmis parviflora, Essential oil, Meloidogyne incognita, Sitophilus zeamais, Tribolium castaneum, Contact toxicity